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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant function in promoting mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two extra splice variants had been described but in-depth nucleotide sequence data isn't still obtainable.

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Blocking peptides are peptides that bind specifically into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.

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This information outlines further achievable optimization of mobile and immunoassay parameters to be certain the best possible final results are...

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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best results are...

Antibodies sure to the blocking peptide no more bind for the epitope about the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.

Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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